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KMID : 0854720080280040292
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Korean Journal of Asthma, Allergy and Clinical Immunology
2008 Volume.28 No. 4 p.292 ~ p.297
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Effects of Co-treatment with Dexamethasone and Troglitazone on Transcription and Secretion of IL-8 in the A549 Airway Epithelial Cells
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Kim Nam-Hee
Lee Ho-Yeon
Seo Ji-Hyun
Hahn Sung-Hwhan
Kim Do-Hyung
Kim Youn-Seup
Park Jae-Seuk
Jee Young-Koo
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Abstract
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Background: Glucocorticoid is a widely used anti-inflammatory agent in a variety of diseases. Recently, peroxisome proliferator activated receptor gamma (PPAR gamma) ligand has been shown to exert a potential anti-inflammatory activity, mainly through their ability to down- regulate pro-inflammatory gene expressions.
Objective: This study was designed to evaluate the effects of co-treatment with dexamethasone and troglitazone (a ligand of PPAR gamma) on IL-8 transcription and secretion.
Method: Steroid and troglitazone-induced transactivation was measured by using pGRE luciferase reporter gene in the A549 cell line. Steroid-induced transrepression was measured by using stable A549 IgG-NF kappa B luciferase cell line, which contained the minimal IL-8 promoter region.
Results: Co-treatment with dexamethasone and troglitazone showed no additional increase of transactivation activity compared to dexamethasone single treatment. Suppressions of the transcriptional activity in the IL-8 promotor and IL-8 secretion showed no difference in the co-treatment with dexamethasone and troglitazone compared to those in dexamethasone single treatment.
Conclusions: Our results suggest that co-treatment with PPAR gamma ligand and steroid may not have an additive anti- inflammatory activity at least in the airway epithelial cell.
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KEYWORD
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Steroid, Peroxisome proliferator activated receptor γ, Troglitazone
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